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permeabilisation buffer inside perm  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec permeabilisation buffer inside perm
    Permeabilisation Buffer Inside Perm, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 348 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeabilisation+buffer+inside+perm/Inside+Stain+Kit/pm40348476-68-5-14
    Average 97 stars, based on 348 article reviews
    permeabilisation buffer inside perm - by Bioz Stars, 2026-09
    97/100 stars

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    Staining:

    Article Title: A pilot study evaluating the feasibility of enriching and detecting circulating tumour cells from peripheral and ovarian veins in rare epithelial ovarian carcinomas.
    Article Snippet: Following the enrichment step, the cells were fixed with 4 % paraformaldehyde (Sigma Aldrich, Ireland) and stained with an antibody cocktail containing anti-epithelial cell adhesion molecule (EpCAM) (9C4, Alexa Fluor 488, BioLegend, USA), antipan-cytokeratin (panCK) (CK3-6H5, FITC, Miltenyi Biotec, Germany), and 20 mM Hoechst 33342 nuclear dye (815-968-0747, Thermo Scientific) for positive selection, and anti-CD45 (HI30, Alexa Fluor 647, BioLegend, USA) to exclude leukocytes. .. All antibodies were diluted in permeabilisation buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec, Germany) at 1:120 dilution. .. Parsortix® cassettes were viewed using EVOS® FL digital inverted fluorescence microscope (Invitrogen™) and CTCs were enumerated as described previously [20].

    Magnetic Cell Separation:

    Article Title: A pilot study evaluating the feasibility of enriching and detecting circulating tumour cells from peripheral and ovarian veins in rare epithelial ovarian carcinomas.
    Article Snippet: Following the enrichment step, the cells were fixed with 4 % paraformaldehyde (Sigma Aldrich, Ireland) and stained with an antibody cocktail containing anti-epithelial cell adhesion molecule (EpCAM) (9C4, Alexa Fluor 488, BioLegend, USA), antipan-cytokeratin (panCK) (CK3-6H5, FITC, Miltenyi Biotec, Germany), and 20 mM Hoechst 33342 nuclear dye (815-968-0747, Thermo Scientific) for positive selection, and anti-CD45 (HI30, Alexa Fluor 647, BioLegend, USA) to exclude leukocytes. .. All antibodies were diluted in permeabilisation buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec, Germany) at 1:120 dilution. .. Parsortix® cassettes were viewed using EVOS® FL digital inverted fluorescence microscope (Invitrogen™) and CTCs were enumerated as described previously [20].



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    97
    Miltenyi Biotec permeabilisation buffer inside perm
    Permeabilisation Buffer Inside Perm, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeabilisation+buffer+inside+perm/Inside+Stain+Kit/pm40348476-68-5-14
    Average 97 stars, based on 1 article reviews
    permeabilisation buffer inside perm - by Bioz Stars, 2026-09
    97/100 stars
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